Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Aug 26;13(16):5895-906.
doi: 10.1093/nar/13.16.5895.

Complete nucleotide sequence of the lipase gene from Staphylococcus hyicus cloned in Staphylococcus carnosus

F Götz, F Popp, E Korn, K H Schleifer
Free PMC article

Complete nucleotide sequence of the lipase gene from Staphylococcus hyicus cloned in Staphylococcus carnosus

F Götz et al. Nucleic Acids Res. .
Free PMC article

Abstract

The lipase gene from Staphylococcus hyicus subsp. hyicus was cloned in Staphylococcus carnosus and Escherichia coli. In both host organisms the lipase gene is expressed and the enzyme is released to the medium. The cloned DNA insert is 2.5 kb in length and DNA sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence. The open reading frame comprises 1923 nucleotides and gives a preprotein of 641 amino acids with a predicted Mr of 71.382. At the 3' end of the structural gene there are three consecutive stop codons and there is also a transcriptional termination signal.

PubMed Disclaimer

References

    1. J Mol Biol. 1969 May 14;41(3):459-72 - PubMed
    1. J Bacteriol. 1965 Apr;89:949-53 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 - PubMed
    1. J Cell Biol. 1975 Dec;67(3):835-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jan;75(1):50-3 - PubMed

Publication types

Associated data