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. 2018 Aug 27;62(9):e00688-18.
doi: 10.1128/AAC.00688-18. Print 2018 Sep.

TEM-184, a Novel TEM-Derived Extended-Spectrum β-Lactamase with Enhanced Activity against Aztreonam

Alessandra Piccirilli  1 , Mariagrazia Perilli  1 , Gianfranco Amicosante  1 , Viola Conte  2 , Carlo Tascini  3 , Gian Maria Rossolini  4   5 , Tommaso Giani  6
Affiliations

TEM-184, a Novel TEM-Derived Extended-Spectrum β-Lactamase with Enhanced Activity against Aztreonam

Alessandra Piccirilli et al. Antimicrob Agents Chemother. .

Abstract

TEM-184, a novel TEM-derived extended-spectrum β-lactamase (ESBL), was isolated from an Escherichia coli ST354 clinical strain. Compared to TEM-1, TEM-184 contains the mutations Q6K, E104K, I127V, R164S, and M182T. Kinetic analysis of this enzyme revealed extended-spectrum activity against aztreonam in particular. TEM-184 was also susceptible to inhibitors, including clavulanic acid, tazobactam, and avibactam.

Keywords: ESBL; avibactam; inhibitors.

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Figures

FIG 1
FIG 1
Sequence alignment of TEM-184 with sequences of TEM-87 (accession no. AF250872), TEM-107 (accession no. AY101764), and TEM-149 (accession no. DQ369751). Top, secondary structure annotation of TEM-1 (PDB-ID 1M40). Arrows, β-strands; spirals, α-helices; red boxes, conserved position; red letters, similar residues. Aligned sequence was made using EsPrit (v.3.0).
See this image and copyright information in PMC

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