Oxytocin response to controlled dietary sodium and angiotensin II among healthy individuals

Abstract

Oxytocin, while classically known for its role in parturition, lactation, and social behavior, also has been implicated in the control of sodium homeostasis in animal models. To improve our understanding of oxytocin physiology in humans, we measured basal oxytocin levels under low- and liberal-dietary-sodium conditions and following a peripheral angiotensin II (ANG II) infusion. Ten healthy individuals underwent a 6-day standardized low-sodium diet and a 6-day liberal-sodium diet. Each diet was followed by a graded ANG II infusion for 30-min sequential intervals at doses of 0.3, 1.0, and 3.0 ng·kg^-1·min^-1. Fasting serum oxytocin was assessed before and after ANG II infusion. Basal oxytocin levels (1,498.5 ± 94.7 vs. 1,663.3 ± 213.9 pg/ml, P = 0.51) did not differ after the low- and liberal-sodium diets. Following the ANG II infusion, ANG II levels and mean arterial pressure significantly increased as expected. In contrast, the ANG II infusion significantly lowered oxytocin levels from 1,498.5 ± 94.7 vs. 1,151.7 ± 118.1 pg/ml ( P < 0.001) on the low-sodium diet and from 1,663.3 ± 213.9 vs. 1,095.2 ± 87.4 pg/ml ( P = 0.03) on the liberal-sodium diet. The percent change in oxytocin following the ANG II infusion did not differ by sodium diet (-25 ± 5% vs. -28 ± 7% low- vs. liberal-sodium conditions, P > 0.99). Dietary sodium intake did not affect circulating oxytocin levels among healthy individuals. Systemic oxytocin levels were significantly suppressed following a peripheral ANG II infusion independent of dietary sodium conditions.

Keywords: angiotensin II; dietary sodium; oxytocin.

Fig. 1.

Oxytocin levels at baseline and following the 90-min angiotensin II (ANG II) infusion during both the low (A)- and liberal (B)-sodium conditions. Dots represent values for each individual subject. P value reported for change in oxytocin from baseline to 90 min among the entire group.

Fig. 2.

Percent change in oxytocin for each individual during the low- and liberal-sodium diets in response to the angiotensin II (ANG II) infusion. The ANG II infusion was terminated for one individual whose blood pressure exceeded the safety limits during the liberal-sodium visit (*).