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. 1977 May;130(2):954-6.
doi: 10.1128/jb.130.2.954-956.1977.

Genetic analysis of Azotobacter vinelandii mutant strains unable to fix nitrogen

P E Bishop, W J Brill

Genetic analysis of Azotobacter vinelandii mutant strains unable to fix nitrogen

P E Bishop et al. J Bacteriol. 1977 May.

Abstract

Transformation was used to perform ratio test crosses with mutant strains of Azotobacter vinelandii unable to fix N2. Mutations that simultaneously eliminated both components of nitrogenase (nif-1 and nif-2) were tightly linked. The nif-45 mutation that resulted in the absence of an active molybdenum cofactor was closer to nif-1 and nif-2 than to any of the other nif mutations. Strains that lacked component I carried mutations that were closely linked to each other. Mutations that probably were located in the structural genes for components I and II appeared to be relatively close to each other on the A. vinelandii genome.

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References

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