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. 2018 Jun 26;23(7):1535.
doi: 10.3390/molecules23071535.

Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma

Kristof Harda  1 , Zsuzsanna Szabo  2 , Erzsebet Szabo  3 , Gabor Olah  4 , Klara Fodor  5 , Csaba Szasz  6 , Gabor Mehes  7 , Andrew V Schally  8   9   10   11 , Gabor Halmos  12   13
Affiliations

Somatostatin Receptors as Molecular Targets in Human Uveal Melanoma

Kristof Harda et al. Molecules. .

Abstract

Uveal melanoma (UM) is the most common primary intraocular malignancy in adults, with an incidence of 4⁻5 cases per million. The prognosis of UM is very poor. In the present study, our aim was to investigate the expression of mRNA and protein for somatostatin receptor types-1, -2, -3, -4, -5 (SSTR-1⁻5) in human UM tissue samples and in OCM-1 and OCM-3 human UM cell lines by qRT-PCR, western blot and ligand competition assay. The mRNA for SSTR-2 showed markedly higher expression in UM tissues than SSTR-5. The presence of SSTRs was demonstrated in 70% of UM specimens using ligand competition assay and both human UM models displayed specific high affinity SSTRs. Among the five SSTRs, the mRNA investigated for SSTR-2 and SSTR-5 receptors was strongly expressed in both human UM cell lines, SSTR-5 showing the highest expression. The presence of the SSTR-2 and SSTR-5 receptor proteins was confirmed in both cell lines by western blot. In summary, the expression of somatostatin receptors in human UM specimens and in OCM-1 and OCM-3 human UM cell lines suggests that they could serve as a potential molecular target for therapy of UM using modern powerful cytotoxic SST analogs targeting SSTR-2 and SSTR-5 receptors.

Keywords: cancer therapy; human uveal melanoma; molecular targets SSTR-1-5; somatostatin receptors.

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Conflict of interest statement

The authors declare no conflicts of interests in this work. The founding sponsors had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, and in the decision to publish the results.

Figures

Figure 1
Figure 1
A representative figure of the expression of SSTR-2 in human uveal melanoma. L: 100 bp DNA Ladder; “−”: negative control; “+”: positive control (UM pool); 1–5: representative human uveal melanoma tissue samples; 6: CACO-2 cell line; 7: OCM-1 human uveal melanoma cell line; 8: OCM-3 human uveal melanoma cell line.
Figure 2
Figure 2
A representative figure of the expression of SSTR-5 in human uveal melanoma. L: 100 bp DNA Ladder; 1–6: representative human uveal melanoma tissue samples; 7: CACO-2 cell line; 8: OCM-1 human UM cell line; 9: OCM-3 human UM cell line. “+”: positive control (UM pool); “−”: negative control.
Figure 3
Figure 3
Expression of SSTR-1-5 in OCM-1, OCM-3 human uveal melanoma cell lines and in CACO-2 human Caucasian colon adenocarcinoma cell lines. qRT-PCR results show that SSTR-1, -2, -3, -4, -5 are expressed in both human UM cell lines examined. CACO-2 cell line was used as a positive control. The p value obtained by GraphPad Prism—one-way ANOVA test was considered to be significant (* p < 0.0001).
Figure 4
Figure 4
Western blot analysis of SSTR-2 and SSTR-5 protein in human cell lines.
See this image and copyright information in PMC

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