Hymenobacter jeollabukensis sp. nov., isolated from soil

Abstract

A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterial strain, designated 1-3-3-8^T, was isolated from soil and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 1-3-3-8^T belongs to the family Cytophagaceae of phylum Bacteroidetes and is most closely related to Hymenobacter paludis KBP-30^T (96.8% similarity), Hymenobacter ocellatus Myx2105^T (96.8%), Hymenobacter coalescens WW84^T (95.6%), and Hymenobacter deserti ZLB-3^T (95.4%). The G + C content of the genomic DNA of strain 1-3-3-8^T was 63.6 mol%. The isolate contained C_15:0 iso (28.4%), summed feature 4 (C_17:1 anteiso B/C_17:1 iso I; 18.9%), and C_15:0 anteiso (17.6%) as major fatty acids, MK-7 as the predominant respiratory quinone, and sym-homospermidine as the predominant polyamine. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The phenotypic and chemotaxonomic data supported the affiliation of strain 1-3-3-8^T with the genus Hymenobacter. The DNA-DNA relatedness between strain 1-3-3-8^T and H. paludis KCTC 32237^T and H. ocellatus DSM 11117^T were 24.5 and 27.4% respectively, clearly showing that the isolate is not related to them at the species level. Overall, the novel strain could be differentiated from its phylogenetic neighbors on the basis of several phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 1-3-3-8^T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter jeollabukensis sp. nov. has been proposed. The type strain is 1-3-3-8^T (= KCTC 52741^T = JCM 32192^T).

Keywords: Bacteroidetes; Hymenobacter; soil bacteria.