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. 2018:145:91-106.
doi: 10.1016/bs.mcb.2018.03.016. Epub 2018 Apr 22.

Live imaging of cell division in 3D stem-cell organoid cultures

Ana C F Bolhaqueiro  1 , Richard H van Jaarsveld  1 , Bas Ponsioen  2 , René M Overmeer  3 , Hugo J Snippert  4 , Geert J P L Kops  5
Affiliations

Live imaging of cell division in 3D stem-cell organoid cultures

Ana C F Bolhaqueiro et al. Methods Cell Biol. 2018.

Abstract

Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.

Keywords: Aneuploidy; Chromosome; Mitosis; Organoid; Stem cell; Tumor.

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