[Structuro-functional organization of the SV40 virus chromosome. IV. Specific organization of regulatory sequences of the SV40 genome in the mini-chromosome]
[Article in
Russian]
- PMID: 2995792
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[Structuro-functional organization of the SV40 virus chromosome. IV. Specific organization of regulatory sequences of the SV40 genome in the mini-chromosome]
[Article in
Russian]
Mol Biol (Mosk).
1985 Jul-Aug.
Abstract
Using previously described technique of hybridization end-labeling, we analysed nucleosomal organization of the regulatory region of SV40 minichromosome. We showed that DNAase II, in spite of certain specificity observed on the naked DNA, cut the minichromosome in a highly specific manner with the major hypersensitive site inside the enhancer. This hypersensitivity and that to micrococcal nuclease were not found when the chromosome of mature SV40 virions was tested.
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