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. 1985 Sep;317(6034):230-4.
doi: 10.1038/317230a0.

Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination

O Smithies, R G Gregg, S S Boggs, M A Koralewski, R S Kucherlapati

Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination

O Smithies et al. Nature. 1985 Sep.

Abstract

A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.

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