doi: 10.1038/317230a0.
Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination
- PMID: 2995814
- DOI: 10.1038/317230a0
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Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination
Nature.
1985 Sep.
Abstract
A 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. The planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed.
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