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Review
. 2018 Jun 28;9(7):326.
doi: 10.3390/genes9070326.

Heterologous Production of Flavour and Aroma Compounds in Saccharomyces cerevisiae

Dariusz R Kutyna  1 , Anthony R Borneman  2
Affiliations
Review

Heterologous Production of Flavour and Aroma Compounds in Saccharomyces cerevisiae

Dariusz R Kutyna et al. Genes (Basel). .

Abstract

Over the last two decades, rapid progress in the field of synthetic biology has opened several avenues for the heterologous de novo production of complex biological compounds, such as biofuels, pharmaceuticals, and food additives in microbial hosts. This minireview addresses the usage of the yeast Saccharomyces cerevisiae as a microbial cell factory for the production of flavour and aroma compounds, thereby providing a path towards a sustainable and efficient means of producing what are normally rare, and often expensive plant-derived chemicals.

Keywords: Saccharomyces cerevisiae; genetic engineering; heterologous production of flavour and aroma compounds; synthetic biology.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Simplified central metabolism of Saccharomyces cerevisiae (blue arrows), and heterologous enzymatic reactions (green arrows) leading to production of various flavour and aroma compounds: vanillin-glucoside (3DSD: 3-dehydroshikimate dehydratase, ACAR: aryl carboxylic acid reductase, OMT: O-methyltransferase, UGT: UDP-glycosyltransferase), raspberry ketone (PAL: phenylalanine/tyrosine ammonia lyase, C4H: cinnamate-4-hydroxlase, 4CL2: coumarate-CoA ligase 2, BAS: benzalacetone synthase), cinnamaldehycde (PAL2: phenylalanine ammonia lyase 2, ACAR: aryl carboxylic acid reductase, EntD: phosphopantetheinyl transferase), geraniol (GES: geraniol synthase), limonene (LMS: limonene synthase), linalool (LIS: linalool synthase), sabinene (SAS: sabinene synthase), valencene (VS: valencene synthase, mtPts: mitochondria targeted valencene synthase), (+)-nootkatone, trans-nootkatol, cis-nootkatol (VOX: (+)-valencene oxidase), nerolidol (NES: nerolidol synthase), β-ionone (crtYB: phytoene synthase/lycopene cyclase, crtI: phytoene desaturase, CCD: carotenoid cleavage dioxygenase), steviol glycosides (CDPS: copalyl diphosphate synthase, KS: kaurene synthase, KO: kaurene oxidase, KAH: kaurenoic acid-13-hydroxylase, UGT: UDP-glycosyltransferase).
See this image and copyright information in PMC

References

    1. Galanie S., Thodey K., Trenchard I.J., Filsinger Interrante M., Smolke C.D. Complete biosynthesis of opioids in yeast. Science. 2015;349:1095–1100. doi: 10.1126/science.aac9373. - DOI - PMC - PubMed
    1. Turner T.L.K.H., Kong I.I., Liu J.J., Zhang G.C., Jin Y.S. Engineering and Evolution of Saccharomyces Cerevisiae to Produce Biofuels and Chemicals. Springer International Publishing; Cham, Switzerland: 2018. pp. 175–215. - PubMed
    1. Teo W.S., Ling H., Yu A.-Q., Chang M.W. Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid short- and branched-chain alkyl esters biodiesel. Biotechnol. Biofuels. 2015;8:177. doi: 10.1186/s13068-015-0361-5. - DOI - PMC - PubMed
    1. Ledesma-Amaro R., Nicaud J.-M. Metabolic engineering for expanding the substrate range of Yarrowia lipolytica. Trends Biotechnol. 2016;34:798–809. doi: 10.1016/j.tibtech.2016.04.010. - DOI - PubMed
    1. Choi K.R., Shin J.H., Cho J.S., Yang D., Lee S.Y. Systems metabolic engineering of Escherichia coli. EcoSal Plus. 2016;7 doi: 10.1128/ecosalplus.ESP-0010-2015. - DOI - PMC - PubMed