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. 1985 Sep 11;13(17):6237-48.
doi: 10.1093/nar/13.17.6237.

The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system

P Kownin, C T Iida, S Brown-Shimer, M R Paule
Free PMC article

The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system

P Kownin et al. Nucleic Acids Res. .
Free PMC article

Abstract

The DNA sequences required for faithful initiation of ribosomal RNA transcription were determined. BAL-31 digestion was used to modify the rDNA template by introducing deletions from its 3'- and 5'-ends. The resulting mutant DNAs were tested for template activity individually or in competition with wild type utilizing an in vitro transcription system from Acanthamoeba castellanii. The results identify the sequence extending from -31 to +8 to be absolutely required for transcription. In addition; when the region between -47 and -32 is left intact, transcription is augmented.

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