Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2018 Jul;33(4):649-659.
doi: 10.3904/kjim.2018.198. Epub 2018 Jun 28.

Applications of kidney organoids derived from human pluripotent stem cells

Yong Kyun Kim  1   2 , Sun Ah Nam  1   2 , Chul Woo Yang  2   3
Affiliations
Review

Applications of kidney organoids derived from human pluripotent stem cells

Yong Kyun Kim et al. Korean J Intern Med. 2018 Jul.

Abstract

The establishment of protocols to differentiate kidney organoids from human pluripotent stem cells provides potential applications of kidney organoids in regenerative medicine. Modeling of renal diseases, drug screening, nephrotoxicity testing of compounds, and regenerative therapy are attractive applications. Although much progress still remains to be made in the development of kidney organoids, recent advances in clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated system 9 (Cas9) genome editing and three-dimensional bioprinting technologies have contributed to the application of kidney organoids in clinical fields. In this section, we review recent advances in the applications of kidney organoids to kidney disease modelling, drug screening, nephrotoxicity testing, and regenerative therapy.

Keywords: Clustered regularly interspaced short palindromic repeats; Kidney; Organoids; Transplantation; Bioprinting.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Figure 1.
Figure 1.
Schematic presentation of the clinical application of kidney organoids derived from human pluripotent stem cells. CRISPR, clustered regularly interspaced short palindromic repeat.
Figure 2.
Figure 2.
Representative images immunohistochemical staining images (with human nuclear antibody [HNA]) after transplantation of kidney organoids derived from human pluripotent stem cells (hPSCs) into kidney of nonobese diabetic/severe combined immunodeficiency (NOD-SCID) mouse. Scale bars, 0.1 mm. Transplanted kidney organoids derived from hPSCs grew with nephron-like structures and the cells in transplanted kidney organoids predominantly originated from the human inducible pluripotent stem cells. Black arrowheads indicate the glomerulus-like structure and white arrowheads indicate the tubule-like structure in transplanted graft.
See this image and copyright information in PMC

References

    1. Morizane R, Lam AQ, Freedman BS, Kishi S, Valerius MT, Bonventre JV. Nephron organoids derived from human pluripotent stem cells model kidney development and injury. Nat Biotechnol. 2015;33:1193–1200. - PMC - PubMed
    1. Taguchi A, Kaku Y, Ohmori T, et al. Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. Cell Stem Cell. 2014;14:53–67. - PubMed
    1. Takasato M, Er PX, Becroft M, et al. Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. Nat Cell Biol. 2014;16:118–126. - PubMed
    1. Takasato M, Er PX, Chiu HS, et al. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature. 2015;526:564–568. - PubMed
    1. Kim YK, Refaeli I, Brooks CR, et al. Gene-edited human kidney organoids reveal mechanisms of disease in podocyte development. Stem Cells. 2017;35:2366–2378. - PMC - PubMed

Publication types