Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus
- PMID: 2997141
- PMCID: PMC214350
- DOI: 10.1128/jb.164.2.954-956.1985
Chromosomal and plasmid locations for phosphoribulokinase genes in Alcaligenes eutrophus
Abstract
Genes coding for phosphoribulokinase (PRK), a key enzyme of the Calvin cycle, were localized in the genome of the chemoautotroph Alcaligenes eutrophus. The NH2-terminal sequence of the PRK subunit was determined. With a synthetic oligodeoxynucleotide probe complementary to a portion of this sequence, hybridization analysis revealed PRK genes to be located on both the chromosome and the megaplasmid pHG1 of A. eutrophus H16.
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