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. 2018:1805:271-289.
doi: 10.1007/978-1-4939-8556-2_14.

DNA Organization and Superesolved Segregation

Diego I Cattoni  1 , Jean-Bernard Fiche  1 , Antoine Le Gall  1 , Marcelo Nollmann  2
Affiliations

DNA Organization and Superesolved Segregation

Diego I Cattoni et al. Methods Mol Biol. 2018.

Abstract

With single-molecule localization microscopy (SMLM) it is possible to reveal the internal composition, architecture, and dynamics of molecular machines and large cellular complexes. SMLM remains technically challenging, and frequently its implementation requires tailored experimental conditions that depend on the complexity of the subcellular structure of interest. Here, we describe two simple, robust, and high-throughput protocols to study molecular motors and machineries responsible for chromosome transport and organization in bacteria using 2D- and 3D-SMLM.

Keywords: Bacillus subtilis; Bacterial chromosome; Molecular motors; ParABS; Photoactivated localization microscopy (PALM); SpoIIIE; Sporulation.

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