Microsatellite loci obtained by next generation sequencing on the sablefish (Anoplopoma fimbria)

Abstract

Eleven microsatellite loci were developed and characterized for the sablefish, Anoplopoma fimbria. The markers were identified from sequences obtained by next generation sequencing. Thirty samples from Aleutians Islands were genotyped. The amplifications were performed with three different annealing temperature and amplification products were visualized in ABI 3500 Genetic Analyzer. No evidence for scoring errors was detected by stuttering or due large allele dropout and neither of the loci presented a high null allele frequency (> 0.2). The number means of alleles per locus was of 12.21 and mean of observed and expected heterozygosity were of 0.60 and 0.75 respectively. The sablefish represents a resource of high commercial value on the coasts of Japan, Russia, Canada and west coast of the United States and these new primers could be useful to future diversity and structure population studies.

Keywords: Genetic diversity; Microsatellites; Next generation sequencing; Sablefish.