Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Oct 25;13(20):7171-82.
doi: 10.1093/nar/13.20.7171.

Purification of Mbo II methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences

M McClelland, M Nelson, C R Cantor
Free PMC article

Purification of Mbo II methylase (GAAGmA) from Moraxella bovis: site specific cleavage of DNA at nine and ten base pair sequences

M McClelland et al. Nucleic Acids Res. .
Free PMC article

Abstract

The restriction modification methylase M. Mbo II has been purified using a sensitive oligonucleotide linker assay. The enzyme methylates the Mbo II recognition sequence* GAAGA at adenine to produce GAAGmA. M. Mbo II can be used in conjunction with the methylation dependent restriction endonuclease Dpn I (GmATC) to produce cleavage at the 10 base sequence GAAGATCTTC. When M. Mbo II is used in combination with M. Cla I (ATCGATCGAT), cleavage by Dpn I occurs at the four ten base sequences GAAGATCTTC, GAAGATCGAT, ATCGATCTTC and ATCGATCGAT, which is equivalent to a nine base recognition site. The use of combinations of adenine methylases and Dpn I to generate highly selective DNA cleavages at a variety of sequences up to fourteen base pairs is discussed.

PubMed Disclaimer

References

    1. Cell. 1981 Oct;26(2 Pt 2):167-80 - PubMed
    1. J Biochem. 1980 Sep;88(3):737-47 - PubMed
    1. Nucleic Acids Res. 1981 Dec 21;9(24):6795-804 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Feb;81(4):983-7 - PubMed
    1. Cell. 1984 May;37(1):67-75 - PubMed

Publication types

MeSH terms

Substances