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. 2017 Apr 4;61(1):97-102.
doi: 10.1515/jvetres-2017-0012. eCollection 2017 Mar.

Serum Paraoxonase as an Indicator for Fatty Liver in Sheep

Yu Cao  1 , Jiang Zhang  1 , Wei Yang  1 , Cheng Xia  1 , Hong-You Zhang  1 , Yan-Hui Wang  1 , Chuang Xu  1
Affiliations

Serum Paraoxonase as an Indicator for Fatty Liver in Sheep

Yu Cao et al. J Vet Res. .

Abstract

Introduction: A model of fatty liver in postpartum sheep was established to measure blood paraoxonase 1 (PON1) and other biochemical indicators, which were used to predict fatty liver in sheep.

Material and methods: Sheep were assigned into two experimental groups: a fatty liver group (T, n = 10) and a healthy control group (C, n = 5). PON1 enzyme activity towards paraoxon as a substrate was quantified spectrophotometrically. The results were analysed by t-test and pearson correlation coefficient. Disease was predicted by binary logistic analysis, and diagnostic thresholds were determined by receiver operatingcharacteristic (ROC) analysis.

Results: The activity of serum PON1 in group T was significantly decreased (P < 0.05) when compared with C group, and liver lipid content and the levels of serum BHBA, NEFA, and TG were significantly increased (P < 0.05). Thresholds were lower than 74.0 U/mL for PON1, higher than 0.97 mmol/L for β-hydroxybutyrate, higher than 1.29 mmol/L for non-esterified fatty acids, higher than 0.24 mmol/L for triglycerides, and lower than 71.35 g/L for total protein.

Conclusion: This study verified that PON1, BHBA, NEFA, TG, and TP could be used to predict the risk of fatty liver in sheep.

Keywords: biochemical indices; fatty liver; paraoxonase 1; sheep.

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Conflict of interest statement

Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

Figures

Fig. 1
Fig. 1
Receiver operating characteristic (ROC) plots for PON1 activity, BHBA, NEFA, TG and TP for diagnosis of ketosis in sheep (PON1: paraoxonase-1; BHBA: hydroxybutyric acid; NEFA: non-esterified fatty acids; TG: triglyceride; TP: total protein). The NEFA line and the TG line coincide together, and part of the BHBA line coincides with the TG line
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