Size and map locations of early transcription products on the Autographa californica nuclear polyhedrosis virus genome

Abstract

The size classes and map locations of early transcripts of Autographa californica nuclear polyhedrosis virus were identified by probing Northern blots with cloned viral DNA fragments. Twelve abundant and 30 minor transcripts were detected by 4 hr postinfection. There was an increase in the abundancy and number of virus-specific transcripts between 6 and 8 hr postinfection, coincident with the onset of DNA replication. The change in transcription pattern did not occur in cells infected at the nonpermissive temperature with a DNA-negative mutant indicating that these early transcripts were synthesized prior to the initiation of viral DNA replication. Two early transcripts, 4.6 and 1.1 kb, mapped to the genomic region of the temperature-sensitive lesion, between 60.1 and 62.0%.