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. 2018:1816:207-220.
doi: 10.1007/978-1-4939-8597-5_16.

Isoproterenol-Induced Heart Failure Mouse Model Using Osmotic Pump Implantation

Sunny C Chang  1 , Shuxun Ren  1 , Christoph D Rau  1 , Jessica J Wang  2
Affiliations

Isoproterenol-Induced Heart Failure Mouse Model Using Osmotic Pump Implantation

Sunny C Chang et al. Methods Mol Biol. 2018.

Abstract

Isoproterenol is used widely for inducing heart failure in mice. Isoproterenol is a nonselective beta-adrenergic agonist. The acute model mimics stress-induced cardiomyopathy. The chronic model mimics advanced heart failure in humans. In this chapter, we describe a protocol that we used to induce heart failure in 100+ strains of inbred mice. Techniques on surgical pump implantation and echocardiography are described in detail. We also discuss the impact of drug dosage, duration, mortality, age, gender, and strain on cardiac remodeling responses. The success of model creation may be assessed by echocardiogram or molecular markers. This chapter may be relevant to those who are interested in using this heart failure model.

Keywords: Cardiac remodeling; Echocardiography; Heart failure; Isoproterenol; Mouse model; Mouse strains.

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Figures

Fig. 1
Fig. 1
Acute and chronic isoproterenol models
Fig. 2
Fig. 2
Variation in echocardiographic measures of cardiac structure and function among HMDP mouse strains. Black bars represent measurements under the baseline condition in ranked order. White bars represent measurements after 3 weeks of continuous ISO infusion. IVSd Interventricular septal wall thickness, LVIDd Left ventricular diastolic diameter, LVM Left ventricular mass, FS Fractional shortening. Error bars represent the standard errors of the means
Fig. 3
Fig. 3
The changes in echocardiographic measures compared to baseline at each ISO time point for individual classical inbred strains
Fig. 4
Fig. 4
Distribution of cardiac fibrosis before and after isoproterenol treatment in the HMDP. Data is organized based on the amount of fibrosis observed after isoproterenol treatment and displays mean +/ standard deviation
See this image and copyright information in PMC

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