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. 2018 May 10;3(3):341-346.
doi: 10.1016/j.bioactmat.2018.05.001. eCollection 2018 Sep.

Combination types between graphene oxide and substrate affect the antibacterial activity

Jiajun Qiu  1   2 , Lu Liu  1   3 , Hongqin Zhu  1 , Xuanyong Liu  1
Affiliations

Combination types between graphene oxide and substrate affect the antibacterial activity

Jiajun Qiu et al. Bioact Mater. .

Erratum in

Abstract

Duo to their superior physicochemical properties, graphene and its derivatives (GDs), such as graphene oxide (GO) and reduced graphene oxide (rGO), have attracted extensive research interests around the world. In recent years, antibacterial activities of GDs have aroused wide concern and substantial works have been done. However, the underlying antibacterial mechanisms still remain controversial. Antibacterial activities of GDs vary with various factors, such as size, number of layers, oxygen-containing groups, and experimental surroundings. We assume that combination types between graphene oxide and substrate may affect the antibacterial activity. Therefore, in this work, GO was fixed on the titanium surface with three kinds of combination types including drop with gravitational effects (GO-D), electrostatic interaction (GO-APS) and electrophoretic deposition (GO-EPD), and the antibacterial activities in vitro were systematically investigated. Results showed that combination types affected the ability of GO for preventing Staphylococcus aureus (S. aureus) from gathering, sharpness of wrinkles or edges and reactive oxygen spices (ROS) levels. Once S. aureus are in the form of separation without aggregation, GO can effectively interact with them and kill them with sharp wrinkles or edges and high ROS levels. GO-EPD could effectively prevent S. aureus from gathering, own sharp wrinkles or edges and could generate higher ROS levels. As a result, GO-EPD exhibited optimal antibacterial activity against S. aureus, followed by GO-APS and GO-D.

Keywords: Antibacterial; Combination type; Graphene oxide; S. aureus; Titanium.

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Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Surface morphologies of Ti, GO-D, GO-APS and GO-EPD. The scale bar is 2 μm.
Fig. 2
Fig. 2
(A) Raman spectra of Ti, GO-D, GO-APS and GO-EPD; (b) XRD patterns of Ti, GO-D, GO-APS and GO-EPD.
Fig. 3
Fig. 3
XPS full spectra of Ti, GO-D, GO-APS and GO-EPD.
Fig. 4
Fig. 4
SEM observation of S. aureus on Ti, GO-D, GO-APS and GO-EPD at different magnification.
Fig. 5
Fig. 5
(A) Bacterial colonies of re-cultivated S. aureus on agar culture plates; (b) bacterial viability of S. aureus on Ti, GO-D, GO-APS and GO-EPD and (c) corresponding antibacterial rate.
Fig. 6
Fig. 6
(A) Inhibition zones around various samples against S. aureus; (b) fluorescence intensity of DCF on various samples.
See this image and copyright information in PMC

References

    1. Chung C., Kim Y.K., Shin D., Ryoo S.R., Hong B.H., Min D.H. Biomedical applications of graphene and graphene Oxide. Acc. Chem. Res. 2013;46(10):2211–2224. - PubMed
    1. Huang X., Zeng Z.Y., Fan Z.X., Liu J.Q., Zhang H. Graphene-based electrodes. Adv. Mater. 2012;24(45):5979–6004. - PubMed
    1. Singh V., Joung D., Zhai L., Das S., Khondaker S.I., Seal S. Graphene based materials: past, present and future. Prog. Mater. Sci. 2011;56(8):1178–1271.
    1. Zhu Y.W., Murali S., Cai W.W., Li X.S., Suk J.W., Potts J.R., Ruoff R.S. Graphene and graphene oxide: synthesis, properties, and applications. Adv. Mater. 2010;22(35):3906–3924. - PubMed
    1. Wu J.S., Pisula W., Mullen K. Graphenes as potential material for electronics. Chem. Rev. 2007;107(3):718–747. - PubMed