Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Aug 31;15(3):82-85.
eCollection 2004 Aug.

Neuroimmunology: Immunoglobulins and the Intrathecal Polyspecific Immune Response in Acute, Subacute and Chronic Neurological Diseases

Hansotto Reiber  1
Affiliations

Neuroimmunology: Immunoglobulins and the Intrathecal Polyspecific Immune Response in Acute, Subacute and Chronic Neurological Diseases

Hansotto Reiber. EJIFCC. .
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Isoelectric focussing on agarose gels with immunoblot: The figure represents the classical types 1 – 5 (Andersson et al. 1994):

  1. Type 1: No bands in CSF and serum.

  2. Type 2: Oligoclonal IgG-bands in CSF, not in serum. Interpretation: Intrathecal IgG-synthesis.

  3. Type 3: Oligoclonal bands in CSF (like type 2) and additional identical oligoclonal bands in CSF and serum (like type 4). Interpretation: Intrathecal IgG-synthesis

  4. Type 4: Identical oligoclonal bands in CSF and serum. Interpretation: No intrathecal IgG-synthesis but systemic immune reaction.

  5. Type 5: Monoclonal bands in CSF and serum. Interpretation: Systemic paraproteinaemia.

Figure 2.
Figure 2.
Isoelectric focussing on polyacrylamidgel with silverstain: In contrast to the immune detection (Fig. 1) we find in the protein stain the albumin range (also place for application of the samples) at about pH = 5.0. In the alcaline range (at pH 9.3) we find cystatin C (gamma-trace-protein) as a single band in CSF (CSF marker).
See this image and copyright information in PMC

References

    1. Reiber H, Peter JB. Cerebrospinal fluid analysis – disease-related data patterns and evaluation programs. J Neurol Sci 2001;184:101-122. - PubMed
    1. Reiber H, Lange P. Quantification of virus-specific antibodies in cerebrospinal fluid and serum: sensitive and specific detection of antibody synthesis in brain. Clin Chem 1991;37:1153-1160. - PubMed
    1. Andersson M, Alvarez-Cermeno J, Bernardi G, Cogato I, Fredman P, Fredriksen J, Fredriksen S, Gallo P, et al. Cerebrospinal fluid in the diagnosis of multiple sclerosis: a consensus report. J Neurol Neurosurg Psychiatr 1994;57:897-902. - PMC - PubMed
    1. Reiber H, Ungefehr St, Jacobi Chr. (1998). The intrathecal, polyspecific and oligoclonal immune response in multiple sclerosis. Multiple Sclerosis 1998;4:111-117 - PubMed
    1. Conrad A J, Chiang E Y, Andeen L E, Avolio C, Walker S M, Baumhefner R W, Mirzayan R, Tourtellotte W W. Quantitation of intrathecal measles virus antibody synthesis rate: subacute sclerosing panencephalitis and multiple sclerosis. J Neuroimmunol 1994;54:99-108. - PubMed

LinkOut - more resources