Two functions of the E protein are key elements in the plasmid F replication control system
- PMID: 2999077
- PMCID: PMC219324
- DOI: 10.1128/jb.164.3.1262-1270.1985
Two functions of the E protein are key elements in the plasmid F replication control system
Abstract
By using a plasmid carrying a translational fusion between the E gene of the IncFI plasmid F and the lacZ gene, we located the operator of the autogenously regulated E gene to an inverted repeat overlapping the E-gene promoter and showing perfect homology to part of the sequence found in all the direct repeats of two regions exerting an inhibitory effect on F replication, incB and incC. Excess E protein provided in trans to an F plasmid increased the replication frequency of the F plasmid. This stimulatory effect was counteracted by increased dosages of incB or incC. A model is proposed for the replication control system of F in which the key elements are autoregulation of E-gene expression and titration of E protein by incB and incC.
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