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. 1985 Dec;164(3):1353-5.
doi: 10.1128/jb.164.3.1353-1355.1985.

lac Up-promoter mutants with increased homology to the consensus promoter sequence

W Mandecki, R A Goldman, B S Powell, M H Caruthers

lac Up-promoter mutants with increased homology to the consensus promoter sequence

W Mandecki et al. J Bacteriol. 1985 Dec.

Abstract

Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.

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References

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