Comparative Study
doi: 10.1093/oxfordjournals.jbchem.a135304.
Cytochrome c oxidase of Pseudomonas AM 1: purification, and molecular and enzymatic properties
- PMID: 2999096
- DOI: 10.1093/oxfordjournals.jbchem.a135304
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Comparative Study
Cytochrome c oxidase of Pseudomonas AM 1: purification, and molecular and enzymatic properties
J Biochem.
1985 Aug.
Free article
Abstract
Cytochrome c oxidase (cytochrome aa3-type) [EC 1.9.3.1] was purified from Pseudomonas AM 1 to an electrophoretically homogeneous state and some of its properties were studied. The oxidase showed absorption peaks at 428 and 598 nm in the oxidized form, and at 442 and 604 nm in the reduced form. The CO compound of the reduced enzyme showed peaks at 432 and 602 nm. The enzyme molecule was composed of two kinds of subunits with molecular weights of 50,000 and 30,000 and it contained equimolar amounts of heme a and copper atom. The enzyme rapidly oxidized Candida krusei and horse ferrocytochromes c as well as Pseudomonas AM 1 ferrocytochrome c. The reactions catalyzed by the enzyme were strongly inhibited by KCN.
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