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. 2018 Jun 4;28(2):7423.
doi: 10.4081/ejtm.2018.7423. eCollection 2018 Apr 24.

Studying the expression rate and methylation of Reprimo gene in the blood of patients suffering from gastric cancer

Amin Abbasi  1 , Sahar Heydari  2
Affiliations

Studying the expression rate and methylation of Reprimo gene in the blood of patients suffering from gastric cancer

Amin Abbasi et al. Eur J Transl Myol. .

Abstract

As gastric cancer has no exclusive signals in its initial phases, it is usually diagnosed in advanced phases. Although many researches have been conducted on methylation and diagnosis of cancer's markers, the methylation and expression of Reprimo gene and its correlation with gastric cancer has not been thoroughly studied. Methylation of Reprimo promoter is a repetitive procedure exclusive to cancer which nullifies its expression and performance. The present research seeks to study the expression and methylation of Reprimo among people suffering with gastric cancer so that it may be used as a biomarker for early diagnosis. Fifty blood samples taken from healthy people (normal samples) and 50 blood samples obtained from gastric cancer patients were analyzed using Real-Time PCR. The methylation status of the promoter of Reprimo was studied using Methylation Specific PCR technique in normal samples and in gastric cancer Iranian patients. We observed reduction in expression rate of Reprimo in the blood samples of patients suffering with gastric cancer in comparison to normal blood samples. A significant correlation was also observed between the expression rate of this gene, age and methylation of its promoter among patients suffering with gastric cancer and various analysis points to a correlation between reduced expressions of Reprimo gene in gastric cancer patients. In conclusion, reduced expression of Reprimo gene and greater levels of methylation of its promoter seems to be promising biomarkers for early diagnosis of gastric cancer.

Keywords: Gastric cancer; MS-PCR; Reprimo; methylation.

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Conflict of interest statement

Conflict of Interest: The authors declare no conflicts of interests.

Figures

Fig 1.
Fig 1.
The melting curve of Reprimo in normal samples and gastric patients
Fig 2.
Fig 2.
The melting curve of GAPDH gene in normal samples and gastric patients
Fig 3.
Fig 3.
Linear curve of Reprimo gene proliferation in normal samples and gastric patients
Fig 4.
Fig 4.
The linear curve of GAPDH gene proliferation in healthy and gastric patients
Fig 5.
Fig 5.
Analysis of Reprimo gene expression in gastric patients compared to normal samples (p-value ≤ 0.0001). 1-35 groups: Average rate of Reprimo gene expression in Gastric cancer Patients. Normal group: Average rate of Reprimo gene expression in normal samples.
Fig 6.
Fig 6.
Analysis of Reprimo gene expression rate in gastric patients compared against normal cases (p ≤ 0.04)
Fig 7.
Fig 7.
Analysis of expression rate of Reprimo gene among cases older than 55 and those younger than 55 (p ≤0.0106)
Fig 8.
Fig 8.
Analysis of expression rate of Reprimo gene among sick cases based upon their gender (p ≤ 0.960))
Fig 9.
Fig 9.
Example of MS-PCR results obtained using methylated primers. The samples with a band size of 158 bp have methylation in the region of Reprimo gene promoter
Fig 10.
Fig 10.
Mean RQ of Reprimo gene based upon methylation status
See this image and copyright information in PMC

References

    1. Sandoval-Bórquez A, Saavedra K, Carrasco-Avino G, Garcia-Bloj B, Fry J, Wichmann I, Corvalán AH. Noncoding genomics in gastric cancer and the gastric precancerous cascade: pathogenesis and biomarkers. Dis Markers 2015; 2015: 503762. - PMC - PubMed
    1. Ikeda F, Doi Y, Yonemoto K, et al. Hyperglycemia increases risk of gastric cancer posed by Helicobacter pylori infection: a population-based cohort study. Gastroenterology 2009;136:1234-41. - PubMed
    1. Baylin SB, Esteller M, Rountree MR, et al. Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer. Hum Mol Genet 2001;10:687-92. - PubMed
    1. Corvalan AH, Garrido M, Maturana MJ, et al. Su1987 Methylated Reprimo in Cell-Free DNA (RPRM) in Comparison With CEA and CA 19-9 As a Tumor Marker in Gastric Cancer. Gastroenterology 2015;148:S-568.
    1. Costello JF, Frühwald MC, Smiraglia DJ, et al. Aberrant CpG-island methylation has non-random and tumour-type-specific patterns. Nat Genet 2000;24:132. - PubMed