Release of progeny virus from cells infected with simian rotavirus SA11

Abstract

Analysis of cells infected with simian rotavirus SA11 at late times of infection indicated that the particles were associated with membranes and the cytoskeleton. Although a large amount of cellular and non-structural viral proteins were released at these times, probably by cellular lysis, only virus with an outer layer was found outside the cells, while virus without an outer layer remained associated with the cells, probably with membranes and the cytoskeleton. Inhibition of glycosylation by tunicamycin did not abolish cell lysis but inhibited the liberation of particles and the non-glycosylated precursors of the structural and non-structural viral glycoproteins. These results indicate that immature virus was tightly associated with the structural matrix of the cell.