Comment
doi: 10.1016/j.tips.2018.06.005.
Epub 2018 Jul 11.
p53 Throws CRISPR a Curve
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- PMID: 30006230
- DOI: 10.1016/j.tips.2018.06.005
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p53 Throws CRISPR a Curve
Trends Pharmacol Sci.
2018 Sep.
Abstract
The efficacy of the powerful CRISPR-Cas9 genome-editing platform depends on DNA repair activities in the cells being targeted. Two new papers show that the low efficiency of targeting in some primary human cell lines is the result of p53-dependent cell arrest in response to the Cas9-induced break. This limitation must be overcome for some anticipated therapies.
Copyright © 2018 Elsevier Ltd. All rights reserved.
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p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells.Nat Med. 2018 Jul;24(7):939-946. doi: 10.1038/s41591-018-0050-6. Epub 2018 Jun 11. Nat Med. 2018. PMID: 29892062
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CRISPR-Cas9 genome editing induces a p53-mediated DNA damage response.Nat Med. 2018 Jul;24(7):927-930. doi: 10.1038/s41591-018-0049-z. Epub 2018 Jun 11. Nat Med. 2018. PMID: 29892067
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