Cloning of mini-mu bacteriophage in cosmids: in vivo packaging into phage lambda heads
- PMID: 3000893
- DOI: 10.1016/0378-1119(85)90107-6
Cloning of mini-mu bacteriophage in cosmids: in vivo packaging into phage lambda heads
Abstract
A technique has been developed that permits the packaging of mini-Mu-carrying cosmids into phage lambda heads. This procedure has several advantages over packaging into Mu helper capsids: the amounts of DNA to be packaged can be increased, the packaging efficiency is improved, and the stability of transducing lysates is high.
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