Corrigendum: Regulation of multiple carbon monoxide consumption pathways in anaerobic bacteria

Abstract

[This corrects the article on p. 147 in vol. 2, PMID: 21808633.].

Keywords: Carboxydothermus hydrogenoformans; CooA; carbon monoxide; carbon monoxide dehydrogenase; carboxydotrophs; hydrogenogens; thermophiles.

Figure 2

Visible spectra of a 2.2 μM (A) CooA-1 and (B) CooA-2. Dotted lines indicate the spectrum of oxidized CooA. Solid line indicates reduced CooA. Dashed line indicates reduced CooA under 1 atm of CO. (C) β-galactosidase activity of CooA-2 expressing E. coli DH5α with a lacZ under the control of the R. rubrum cooF promoter. Experiments with CO were grown with 2% CO in the headspace of the culture.

Figure 4

Electrophoretic mobility shift assays at 25°C. Gel shift results showing the binding of CooA-1 or CooA-2 to either pacs or the pcooC^hyd promters from C. hydrogenoformans. Arrows indicate formation of the CooA-promoter complex. (A) CooA-1 binding to pacs (Protein added: 3, 6, 12, 18 μg/ml). (B) CooA-1 binding to pcooC^hyd (Protein added: 3, 6, 9, 12, 15 μg/ml). (C) CooA-2 binding to pacs (Protein added: 3, 6, 9 μg/ml). (D) CooA-2 binding to pcooC^hyd (Protein added: 3, 6, 9, 12, 15 μg/ml).

The original gel image used to prepare Figure 4.