Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Dec 20;13(24):8729-37.
doi: 10.1093/nar/13.24.8729.

A wheat HMW glutenin subunit gene reveals a highly repeated structure

T Sugiyama, A Rafalski, D Peterson, D Söll
Free PMC article

A wheat HMW glutenin subunit gene reveals a highly repeated structure

T Sugiyama et al. Nucleic Acids Res. .
Free PMC article

Abstract

A wheat genomic library was screened with two synthetic oligonucleotides (24 and 25 bases in length) complementary to a partial cDNA clone encoding a glutenin gene [Thompson et al. (1983) Theor. Appl. Genet. 67, 87-96]. Glutenins are large molecular weight aggregated proteins of grain endosperm, and major determinants of bread making quality of wheat. Of the two clones obtained one was fully characterized. It contained the sequence of the high molecular weight subunit of glutenin. The amino acid sequence derived from the gene sequence reveals a mature protein (817 amino acids) with a highly repeated structure of two different motifs corresponding to the high glutamine (35.7%), glycine (20.1%) and proline (13.1%) content. The gene does not contain an intron, and possesses a typical eukaryotic promoter; the RNA initiation site is 25-30 bases downstream.

PubMed Disclaimer

References

    1. Cell. 1977 Nov;12(3):721-32 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4298-302 - PubMed
    1. EMBO J. 1984 Jun;3(6):1409-15 - PubMed
    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
    1. Nucleic Acids Res. 1983 Aug 25;11(16):5521-40 - PubMed

Publication types

Associated data