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. 2018 Jul 16:26:e20170266.
doi: 10.1590/1678-7757-2017-0266.

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

Mustafa Özay Uslu  1 , Abubekir Eltas  1 , İsmail Marakoğlu  2 , Serkan Dündar  3 , Kazım Şahin  4 , İbrahim Hanifi Özercan  5
Affiliations

Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model

Mustafa Özay Uslu et al. J Appl Oral Sci. .

Abstract

Objective: In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model.

Materials and methods: We used Wistar-Albino rats (n=60) with average weight of 230 g. Experimental periodontitis was induced by ligature at the right and left first mandibular molar teeth in all rats. After 11 days, the ligature was removed and rats were divided into two groups. The control group (n=30) received only SRP treatment, while the laser group (n=30) received a diode laser (GaAlAs, 810 nm, 1 W, 10 J, 20 s) treatment adjunctive to SRP. Ten rats in each group were sacrificed after 7, 15, and 30 days. Histopathological examination was performed in the left mandible of rats. Myeloperoxidase (MPO) was evaluated by western blot in the gingival specimens from the right mandible.

Results: MPO levels in the laser group were statistically significantly lower compared with the control group (p≤0.05). There was no statistically significance at any time between MPO levels in the control group (p>0.05). MPO levels in the laser group at the 7th day were statistically significantly higher compared to the 15th (p≤0.05) and the 30th day (p≤0.05). Inflammatory cell infiltration decreased over time in both groups and was statistically significantly lower in the laser group than in the control group at all times (p≤0.01).

Conclusions: Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues.

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Conflict of interest statement

Disclosure statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1. The experimental study model
Figure 2
Figure 2. Plaque accumulation and induction of periodontal disease on ligatured first molar tooth at 11th day
Figure 3
Figure 3. First molars were submitted to SRP with manual n. 1/2 micro mini five Gracey curettes through 10 distal-mesial traction movements in both buccal and lingual aspects
Figure 4
Figure 4. Interproximal areas were scaled with the same curettes through cervico-occlusal traction movements
Figure 5
Figure 5. The laser was applied to three equidistant points at each buccal and lingual aspect of the mandibular first molar in contact with the tissue
Figure 6
Figure 6. Photomicrograph illustrating the areas of bone loss in the interdental region of the mandibular left first molar with induced periodontal disease in the different groups (control and laser): (A) Control group, scaling and root planing (SRP) treatment for 7 days (H&E, original magnification 40×); (B) Control group (H&E, original magnification 100×); (C) Laser group, administered diode laser adjunctive to SRP treatment for 7 days (H&E, original magnification 40×); (D) Laser group (H&E, original magnification 100×)
Figure 7
Figure 7. Photomicrograph illustrating the areas of bone loss in the interdental region of the mandibular left first molar with induced periodontal disease in the different groups (control and laser): (A) Control group, SRP treatment for 15 days (H&E, original magnification 40×); (B) Control group (H&E, original magnification 100×); (C) Laser group, administered diode laser adjunctive to SRP treatment for 15 days (H&E, original magnification 40×); (D) Laser group (H&E, original magnification 100×)
Figure 8
Figure 8. Photomicrograph illustrating the areas of bone loss in the interdental region of the mandibular left first molar with induced periodontal disease in the different groups (control and laser): (A) Control group, SRP treatment for 30 days (H&E, original magnification 40×); (B) Control group (H&E, original magnification 100×); (C) Laser group, administered diode laser adjunctive to SRP treatment for 30 days (H&E, original magnification 40×); (D) Laser group (H&E, original magnification 100×)
Figure 9
Figure 9. MPO levels in control and laser group. Significant difference among the experimental periods (T1, T2, and T3) in the same group
Figure 10
Figure 10. ICI levels in control and laser group. Significant difference among the experimental periods (T1, T2, and T3) in the same group
Figure 11
Figure 11. Western blotting bands of MPO and actin. A: Control at 7th day, B: Laser at 7th day, C: Control at 15th day, D: Laser at 15th day, E: Control at 30th day, F: Laser at 30th day
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