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. 1986 Feb;165(2):373-8.
doi: 10.1128/jb.165.2.373-378.1986.

Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA

W D Beck, B Berger-Bächi, F H Kayser

Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mec-specific DNA

W D Beck et al. J Bacteriol. 1986 Feb.

Abstract

Additional DNA was shown to be present in methicillin-resistant Staphylococcus aureus by one- and two-dimensional restriction endonuclease analyses of the chromosomal DNA. A 3.5-kilobase Bg/II fragment, which was present in methicillin-resistant strains but not in the isogenic methicillin-sensitive parental strain, was cloned into newly constructed plasmid pWDB1 in Escherichia coli. Hybridization of this 3.5-kilobase Bg/II fragment with different methicillin-sensitive and methicillin-resistant S. aureus clinical isolates indicated that the fragment represents part of the methicillin resistance determinant (mec). In addition, the fragment carries a sequence that is present in some large staphylococcal plasmids, as well as in penicillinase plasmid pI524.

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