Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Feb;165(2):523-6.
doi: 10.1128/jb.165.2.523-526.1986.

Lipopolysaccharide-free Escherichia coli OmpF and Pseudomonas aeruginosa protein P porins are functionally active in lipid bilayer membranes

T R Parr Jr, K Poole, G W Crockford, R E Hancock

Lipopolysaccharide-free Escherichia coli OmpF and Pseudomonas aeruginosa protein P porins are functionally active in lipid bilayer membranes

T R Parr Jr et al. J Bacteriol. 1986 Feb.

Abstract

Escherichia coli porin OmpF and Pseudomonas aeruginosa porin protein P were eluted from sodium dodecyl sulfate-polyacrylamide gels. The resultant porin preparations were found to be devoid of detectable lipopolysaccharide (LPS) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining for LPS, direct enzyme-linked immunosorbent assays with LPS-specific monoclonal antibodies, and 2-keto-3-deoxyoctulosonic acid assays. The average conductances, ionic selectivities and incorporation rates of the electroeluted porins were identical to those of their conventionally purified counterparts. These data suggest that LPS is not required per se for porin function.

PubMed Disclaimer

References

    1. J Biol Chem. 1972 Aug 25;247(16):5123-31 - PubMed
    1. Biochim Biophys Acta. 1979 Mar 8;551(2):238-47 - PubMed
    1. Can J Microbiol. 1979 Mar;25(3):423-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
    1. J Bacteriol. 1979 Dec;140(3):1071-80 - PubMed

Publication types

MeSH terms

LinkOut - more resources