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. 1977 Jan;27(1):38-42.

C3-reacted sepharose: a preparative method for separating T and B lymphocytes

P Casali, B M Perussia

C3-reacted sepharose: a preparative method for separating T and B lymphocytes

P Casali et al. Clin Exp Immunol. 1977 Jan.

Abstract

Lymphocytes bearing complement receptors were separated from lymphocyte suspensions using C3-reacted Sepharose columns as solid phase immunoabsorbents. The retained CR+ lymphocytes were recovered by elution of the column with IgG fraction purified from a rabbit antiserum to human C3. This paper describes the method, its specificity and the yields of cells obtained.

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