Intraurethral Erythropoietin to Prevent Fibrosis and Improve Urethral Healing: An Experimental Study in a Rat Model

Abstract

Objective: To determine the effects of intraurethral erythropoietin (EPO) on an experimentally induced urethral injury in a rat model with respect to wound healing enhancement and the prevention of spongiofibrosis MATERIAL AND METHODS: A urethral injury model was created by traumatizing the urethra of male rats with a tilted-tip insulin injector. Thirty rats were randomly separated into 3 groups of 10; Group 1 (control) received 0.9% saline solution twice a day, Group II received EPO 25 IU/kg once a day and 0.9% saline solution once a day, and Group III received EPO 25 IU/kg twice a day. All applications were made intraurethrally via a 24 ga catheter sheath. To investigate inflammation and spongiofibrosis and congestion of vessels in the lamina propria, the penises of the rats were harvested for histopathologic evaluation after a follow-up period of 14 days.

Results: Histopathologic analysis revealed less fibrosis and inflammation and higher congestion of vessels in Group III that had received high-dose EPO. There was a significant decrease in both spongiofibrosis and inflammation and an increase in congestion in Groups II and III compared to the control group (P = .001, for all). In the comparison of Group II with Group III, no statistically significant differences were found in terms of these 3 parameters (P = .5, P = .6, P = .27, respectively).

Conclusion: The results of this study have shown that EPO has a preventive effect on spongiofibrosis and improve urethral wound healing in a rat model of urethral injury.