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. 2018 Jul 20;23(7):1804.
doi: 10.3390/molecules23071804.

Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

Xiao Chen  1 , Jia Xiong  2 , Lingxiao He  3 , Yu Zhang  4 , Xun Li  5 , Liping Zhang  6 , Fei Wang  7
Affiliations

Effects of In Vitro Digestion on the Content and Biological Activity of Polyphenols from Acacia mearnsii Bark

Xiao Chen et al. Molecules. .

Abstract

The stability and bioaccessibility of polyphenol from Acacia mearnsii bark were measured at various stages during in vitro simulated digestion. Subsequently, the changes in the total polyphenol content (TPC) and biological activity were studied. The results showed that the phenolic compounds from A. mearnsii remained stable, and TPC underwent few changes during gastric digestion. Nonetheless, intestinal digestion led to the degradation of proanthocyanidins (PAs) and a significant decrease in TPC (26%). Degradation was determined by normal-phase HPLC and gel permeation chromatography. Only monomers, dimers, and trimers of flavan-3-ols were identified in the serum-accessible fraction for characterization of their bioaccessibility. The results also indicated the obvious antioxidant capacity of PAs from A. mearnsii bark, and ~53% of the α-glucosidase⁻inhibitory effect was preserved. All these findings show that PAs from A. mearnsii bark as a native plant source may be particularly beneficial for human health as a natural nutritional supplement.

Keywords: Acacia mearnsii; HPLC/MS; antidiabetic; antioxidant; in vitro digestion; proanthocyanidins.

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Conflict of interest statement

There are no potential conflicts of interest to be reported by the authors.

Figures

Figure 1
Figure 1
Representative normal-phase HPLC/VWD chromatograms of samples from A. mearnsii bark at different time points during the simulated gastric-intestinal digestion, (A) gastric digestion; (B) intestinal digestion. The OUT samples represent the solution outside the tubing.
Figure 2
Figure 2
Representative gel permeation chromatograms of the raw extract, gastric digestion at 120 min, and OUT 120 min samples prepared in tetrahydrofuran.
Figure 3
Figure 3
Percent α-glucosidase inhibition for gastric digesta and OUT samples at different time points during in vitro gastrointestinal digestion. The mean values reported at each digestion stage-those with a common superscript (a–c) are not significantly different from each other (p < 0.05). Data are means of three independent determinations ± SD.
See this image and copyright information in PMC

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