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. 2018 Jul 21;23(7):1814.
doi: 10.3390/molecules23071814.

The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats

Aline Boveto Santamarina  1 , Giovana Jamar  2 , Laís Vales Mennitti  3 , Veridiana Vera de Rosso  4 , Helena Cassia Cesar  5 , Lila Missae Oyama  6 , Luciana Pellegrini Pisani  7   8
Affiliations

The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats

Aline Boveto Santamarina et al. Molecules. .

Abstract

Obesity is associated with modern diets that are rich in saturated fatty acids. These dietary patterns are linked to low-grade proinflammatory mechanisms, such as the toll-like receptor 4/nuclear factor kappa-B (NF-κB) pathway rapidly activated through high-fat diets. Juçara is a berry rich in anthocyanins and unsaturated fatty acids, which prevents obesity and associated comorbidities. We evaluated the effect of different doses of freeze-dried juçara pulp on NF-κB pathway after the consumption of short-term high-fat diet. Male Wistar rats with ad libitum access to food and water were divided into four groups: Control diet (C), high-fat diet (HFC), high-fat diet with 0.25% juçara (HFJ 0.25%), and high-fat diet with 0.5% juçara (HFJ 0.5%). Energy intake and body weight gain were increased in HFC and HFJ 0.5% groups compared to C group. The hypothalamus weight reduced in the HFC group compared to C and HFJ 0.25% groups. Cytokines, MYD88, TRAF6, and pNF-κBp50 levels in the hypothalamus, serum triacylglycerol, LDL-cholesterol (LDL-C), and free fatty acid levels were improved in the HFJ 0.25% group. In summary, the HFJ 0.25% group had better protective effects than those in the HFJ 0.5%. Therefore, 0.25% juçara can be used to protect against central inflammation through the high-fat diet-induced NF-κB pathway.

Keywords: NF-κB pathway; hypothalamus; inflammation; juçara; short-term high-fat diet.

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Conflict of interest statement

All authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
(a) Mean daily energy intake estimative during the experiment; (b) body weight gain (Δ). # p < 0.05 compared with the control diet (C) group (n = 6 or 7).
Figure 2
Figure 2
Cytokine levels of protein extract samples from the hypothalamus: (a) TNF-α, (b) Il-10, and (c) IL-6. * p < 0.05 compared with the high-fat juçara 0.25% (HFJ 0.25%) group; # p < 0.05 compared with the control diet (C) group (n = 6 or 7).
Figure 3
Figure 3
Protein expression in the NF-κB pathway in the hypothalamus: (a) TLR4, (b) MYD88, (c) TRAF6, and (d) pNF-κBp50. The housekeeping used for all analyses was β-actin expression. * p < 0.05 compared with the high-fat juçara 0.25% (HFJ 0.25%) group; # p < 0.05 compared with the control diet (C) group (n = 6).
See this image and copyright information in PMC

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