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. 2018 Jul 23;8(1):11069.
doi: 10.1038/s41598-018-29332-7.

The microRNA miR-192/215 family is upregulated in mucinous ovarian carcinomas

Antonio Agostini  1 , Marta Brunetti  1 , Ben Davidson  2   3 , Claes G Tropé  4 , Ane Gerda Z Eriksson  4 , Sverre Heim  1   3 , Ioannis Panagopoulos  1 , Francesca Micci  5
Affiliations

The microRNA miR-192/215 family is upregulated in mucinous ovarian carcinomas

Antonio Agostini et al. Sci Rep. .

Abstract

Different microRNAs are dysregulated in ovarian cancer where some of them have proved to be valid biomarkers. miRNA profiling analyses have shown that the different histotypes of ovarian carcinoma display differential expression of specific miRNAs. In the present study, we used miRNA-sequencing and Real-Time qPCR to detect the expression levels of miRNAs belonging to the miRNA-192/215 family, namely miR-192, miR-194, and miR-215, in different types of ovarian neoplasia, finding that miR-192, miR-194, and miR-215 were upregulated in ovarian carcinomas of the mucinous subtype, but downregulated in other types of carcinoma and in sex cord-stromal tumors. The expression of the said miRNAs was 6-fold higher in mucinous tumors compared to the other histotypes making them candidates for a possible role as diagnostic biomarkers.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
miR-192/215 family expression levels in ovarian tumors assessed by Real-Time qPCR. (A) Normalized relative expression of miR-192 (blue), miR-194 (green), and miR-215 (red) in mucinous carcinomas. (B) Overview of the normalized relative expression of the miR-192/215 family of miRNAs in the whole series analyzed (73 samples). (M) mucinous carcinoma, (ThF) thecofibroma, (F) fibroma, (CC) clear cell carcinoma, (E) endometrioid carcinoma, (LGS) low-grade serous carcinoma, (HGS) high-grade serous carcinoma.
Figure 2
Figure 2
Immunohistochemistry analyses of case XI. (A) Staining for Hematoxylin and Eosin (HE) and immunostaining for PAX8 (negative) (B), CEA (positive) (C), and ER (negative) (D).
See this image and copyright information in PMC

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