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. 1977;15(1):57-66.
doi: 10.1016/0022-1759(77)90017-5.

A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureus

J P Brown, J M Klitzman, K E Hellström

A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureus

J P Brown et al. J Immunol Methods. 1977.

Abstract

A sensitive assay for antibodies bound to the surface antigens of adherent tumor cells using protein A from Staphylococcus aureus is described. Cells, as monolayers in microtest wells, are incubated first with immune or control serum, and then with 125I-labelled protein A (IPA), which binds to IgG antibodies bound to cell surface antigens. The IPA assay is shown to be more sensitive than the conventional isotopic antiglobulin (IAG) assay, because IPA binds to a lesser extent to IgG bound non-specifically to cells.

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