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. 2018 Jul 23;8(3):31.
doi: 10.3390/life8030031.

Global Molecular Diversity of the Halotolerant Fungus Hortaea werneckii

Alessia Marchetta  1   2 , Bert Gerrits van den Ende  3 , Abdullah M S Al-Hatmi  4   5   6 , Ferry Hagen  7 , Polona Zalar  8 , Montarop Sudhadham  9 , Nina Gunde-Cimerman  10 , Clara Urzì  11 , Sybren de Hoog  12   13 , Filomena De Leo  14
Affiliations

Global Molecular Diversity of the Halotolerant Fungus Hortaea werneckii

Alessia Marchetta et al. Life (Basel). .

Abstract

A global set of clinical and environmental strains of the halotolerant black yeast-like fungus Hortaea werneckii are analyzed by multilocus sequencing and AFLP, and physiological parameters are determined. Partial translation elongation factor 1-α proves to be suitable for typing because of the presence/absence of introns and also the presence of several SNPs. Local clonal expansion could be established by a combination of molecular methods, while the population from the Mediterranean Sea water also responds differently to combined temperature and salt stress. The species comprises molecular populations, which in part also differ physiologically allowing further diversification, but clinical strains did not deviate significantly from their environmental counterparts.

Keywords: AFLP; Hortaea werneckii; epidemiology; haplotype networks; multilocus sequencing.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Clustering of the amplified length polymorphism banding pattern of the 67 Hortaea werneckii isolates. The dendrogram is generated using the Unweighted Pair Group Method with Arithmetic Mean algorithm.
Figure 2
Figure 2
Haplotype networking generated on the basis of the SNPs in TEF1 sequence data. Presence/absence of introns and AFLP groups is shown.
See this image and copyright information in PMC

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