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. 2018 Sep;32(9):2016-2020.
doi: 10.1038/s41375-018-0220-z. Epub 2018 Jul 24.

Identification of immune-activated hematopoietic stem cells

Nir Bujanover  1   2 , Oron Goldstein  1   2   3 , Yariv Greenshpan  1 , Hodaya Turgeman  1 , Amit Klainberger  1   2 , Ye'ela Scharff  1   2 , Roi Gazit  4   5   6
Affiliations

Identification of immune-activated hematopoietic stem cells

Nir Bujanover et al. Leukemia. 2018 Sep.
No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Acute and extended immune stimulation provokes conventional HSC markers. a Representative FACS plots, showing the staining of the Lineage−cKit+Sca1+ (LSK) compartment (left panels) and its dissection by CD150 and the Fgd5mCherry reporter (mC, right panels) under control conditions (top) and under acute (middle) and extended (bottom) pIC stimulation, 24 h post stimulation. b Quantification of the frequencies of indicated cell populations: Lineage-cKit+Sca1− (LK), Lineage-cKit+Sca1+ (LSK), LSKCD150+mC−, and LSKCD150+mC+. Histograms indicate mean frequency and standard deviation from the bone-marrow mononuclear cells (% of total). c LSKCD150+mC+ are functional HSCs. Chimerism (% of gated CD45.2 cells) over time following acute or extended pIC-stimulated donors. Data are from at least five mice per histogram; *p < 0.05, **p < 0.01
Fig. 2
Fig. 2
Surface markers of acutely activated HSCs. a A heatmap of 75 differentially expressed (DE) surface markers (GO:0016021, of 435 DE genes) under control (PBS) conditions and 24 h after an acute pIC stimulation. b Representative FACS plots showing the expression of CD69 (x-axis) and CD317 (Bts2, y-axis) in control conditions (PBS; top panels) and 24 h after an acute pIC stimulation (bottom panels). Plots are shown for the Lineage-negative (Lin−, left panels), Lineage−cKit+Sca1+ (LSK, middle panels), and LSKCD150+mCh+ (right panels) populations. c Quantification of the frequencies of indicated populations of CD69+CD317+ (green), CD317+CD69− (red), and CD69+CD317− (blue) cells. Histograms show averages and standard deviations of the control (PBS) and pIC-treated (Acute) cells; *p < 0.05, **p < 0.01
See this image and copyright information in PMC

References

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