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. 2018:1822:71-82.
doi: 10.1007/978-1-4939-8633-0_5.

Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula

Myriam Sánchez  1 , Christine Le Signor  1 , Gregoire Aubert  1 , Brigitte Darchy  1 , Karine Gallardo  1 , Richard D Thompson  2
Affiliations

Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula

Myriam Sánchez et al. Methods Mol Biol. 2018.

Abstract

TILLING is a reverse genetics strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid screening of DNA pools from a mutagenized population for induced mutations (McCallum et al., Nat Biotechnol 18:455-457, 2000). This high-throughput technique allows the identification of point mutations in any gene of interest.

Keywords: EMS chemical mutagenesis; Medicago truncatula; Reverse genetics; TILLING.

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