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. 2018 Sep 25;56(10):e01108-18.
doi: 10.1128/JCM.01108-18. Print 2018 Oct.

Pulmonary Tuberculosis Conversion Documented by Microscopic Staining for Detection of Dynamic, Dormant, and Dead Mycobacteria (DDD Staining)

A Loukil  1 , F Darriet-Giudicelli  1 , C Eldin  1   2 , M Drancourt  3
Affiliations

Pulmonary Tuberculosis Conversion Documented by Microscopic Staining for Detection of Dynamic, Dormant, and Dead Mycobacteria (DDD Staining)

A Loukil et al. J Clin Microbiol. .
No abstract available

Keywords: Mycobacterium tuberculosis; dormancy; fluorescence microscopy; pulmonary tuberculosis.

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Figures

FIG 1
FIG 1
DDD staining results after microscopic observation and counting. DAPI, FDA, and NR channels were used for the microscopic acquisition and image overlay. Image acquisition was performed with a Zeiss spinning-disk confocal microscope using a 63×, 1.4 numeric aperture (NA) oil immersion objective. (A) Comparison between the percentages of dynamic, dead, and dormant Mycobacterium tuberculosis mycobacteria in the sputum specimens collected before and after antituberculous treatment. **, P < 0.001 by Pearson's chi-square statistic test. (B) Fluorescence microscopy images of sputum smears collected before antituberculous treatment showing dynamic mycobacteria as FDA- and DAPI-positive bacilli (green arrows), dead mycobacteria as DAPI-positive and FDA-negative bacilli (blue arrow), and dormant mycobacterium as FDA- and DAPI-positive bacilli holding NR-positive intracellular inclusions (red arrow). (C) Fluorescence microscopy images of sputum smears from specimens collected before antituberculous treatment, showing dynamic mycobacteria as FDA- and DAPI-positive bacilli (green arrow) and a dormant mycobacterium as FDA-negative and DAPI-positive bacilli with NR-positive intracellular lipid bodies (red arrow). (D) Fluorescence microscopy images of sputum smears from specimens collected after antituberculous treatment, showing a dormant M. tuberculosis bacterium holding five NR-positive intracellular inclusions and a dormant M. tuberculosis bacterium measuring 4.4 μm and containing 2 lipid bodies.
See this image and copyright information in PMC

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