Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 Dec 1;4(12):3209-13.
doi: 10.1002/j.1460-2075.1985.tb04067.x.

The 5' region of the p53 gene: evolutionary conservation and evidence for a negative regulatory element

Comparative Study

The 5' region of the p53 gene: evolutionary conservation and evidence for a negative regulatory element

B Bienz-Tadmor et al. EMBO J. .

Abstract

The 5' regions of the mouse, rat and human functional p53 genes were isolated and analysed. All three genes possess a non-coding exon, comprising exclusively 5' untranslated sequences. This exon contains extensive diad symmetry near the 5' end of p53 mRNA, possibly allowing for the formation of a stable hairpin structure in this mRNA. The nucleotide sequence within this hairpin element is highly conserved among the species. A DNA stretch of 225 bp preceding the p53 mRNA cap site possesses distinct promoter activity when assayed in the CAT system. However, this activity is practically abolished when further upstream p53 sequences (approximately 120 bp) are included in front of the CAT gene. This suggests that the control of p53 gene expression is complex and involves a negative regulatory element.

PubMed Disclaimer

References

    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. Mol Cell Biol. 1985 Aug;5(8):1887-93 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed

Publication types