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Comment
. 2018 Aug;20(8):862-863.
doi: 10.1038/s41556-018-0152-x.

Assembling a protective shield

Roger A Greenberg  1
Affiliations
Comment

Assembling a protective shield

Roger A Greenberg. Nat Cell Biol. 2018 Aug.
No abstract available

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Conflict of interest statement

Competing interests

The author declares no competing interests.

Figures

Fig. 1 |
Fig. 1 |. Assembly of the Shieldin complex at DNA DSBs limits generation of single stranded overhangs to promote NHEJ.
The drawing represents a composite model derived from references–, although further studies will be necessary to fully understand the architecture of the Shieldin complex. The Shieldin complex links 53BP1–Rif1 recognition of nucleosomes flanking DSBs to single stranded DNA at the break site. Shieldin may limit end resection by preventing the action of nucleases that resect DNA with a 5’ to 3′ polarity. Additionally, Shieldin is required for localization of the CST complex, which interacts with DNA Polα -primase and could counteract end resecting nucleases by fill-in DNA synthesis on the resected 3′ end of single stranded DNA at break sites. This 53BP1–Rif1–Shieldin–CST pathway is necessary to promote NHEJ during class switch recombination at immunoglobulin loci and following PARP inhibitor treatment of BRCA1 mutant cells. Ub, ubiquitin on lysine 15 of histone H2A; Me2, dimethylation on lysine 20 of histone H4.
See this image and copyright information in PMC

Comment on

  • Shieldin complex promotes DNA end-joining and counters homologous recombination in BRCA1-null cells.
    Dev H, Chiang TW, Lescale C, de Krijger I, Martin AG, Pilger D, Coates J, Sczaniecka-Clift M, Wei W, Ostermaier M, Herzog M, Lam J, Shea A, Demir M, Wu Q, Yang F, Fu B, Lai Z, Balmus G, Belotserkovskaya R, Serra V, O'Connor MJ, Bruna A, Beli P, Pellegrini L, Caldas C, Deriano L, Jacobs JJL, Galanty Y, Jackson SP. Dev H, et al. Nat Cell Biol. 2018 Aug;20(8):954-965. doi: 10.1038/s41556-018-0140-1. Epub 2018 Jul 18. Nat Cell Biol. 2018. PMID: 30022119 Free PMC article.

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