Engineering Clostridium organisms as microbial cell-factories: challenges & opportunities

Kamil Charubin¹, R Kyle Bennett², Alan G Fast³, Eleftherios T Papoutsakis⁴

Affiliations

¹ Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: kamilcha@udel.edu.
² Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: rkbenne@udel.edu.
³ Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: alanfast@udel.edu.
⁴ Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: epaps@udel.edu.

PMID: 30055325
DOI: 10.1016/j.ymben.2018.07.012

Review

Engineering Clostridium organisms as microbial cell-factories: challenges & opportunities

Kamil Charubin et al. Metab Eng. 2018 Nov.

. 2018 Nov:50:173-191.

doi: 10.1016/j.ymben.2018.07.012. Epub 2018 Jul 25.

Authors

Kamil Charubin¹, R Kyle Bennett², Alan G Fast³, Eleftherios T Papoutsakis⁴

Affiliations

¹ Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: kamilcha@udel.edu.
² Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: rkbenne@udel.edu.
³ Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: alanfast@udel.edu.
⁴ Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy St., Newark, DE 19716, USA; The Delaware Biotechnology Institute, Molecular Biotechnology Laboratory, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA. Electronic address: epaps@udel.edu.

PMID: 30055325
DOI: 10.1016/j.ymben.2018.07.012

Abstract

Clostridium organisms are of major importance in the development of technologies to produce biofuels and chemicals. They are uniquely capable of utilizing virtually all biomass-derived carbohydrates, as well as waste gases, waste materials, and C1 compounds, and they possess diverse biosynthetic capabilities for producing a broad spectrum of metabolites, including those of C4-C8 chain length. They can also be readily used in synthetic, syntrophic, and other microbial consortia to broaden the biosynthetic repertoire of individual organisms, thus enabling the development of novel biotechnological processes. Engineering Clostridium organisms at the molecular and population level is hampered by genetic engineering, genome engineering, and microbial-population engineering tools. We discuss these challenges, and the promise that derives from their resolution aiming to usher in an era of broader use of Clostridium organisms as biotechnological platforms.

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Engineering Clostridium organisms as microbial cell-factories: challenges & opportunities

Affiliations

Engineering Clostridium organisms as microbial cell-factories: challenges & opportunities

Authors

Affiliations

Abstract

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LinkOut - more resources

Full Text Sources

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