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. 2018 Aug;68(4):300-305.
doi: 10.1007/s13224-017-0984-5. Epub 2017 Mar 29.

Frequency of Red Cell Alloantibodies in Pregnant Females of Navsari District: An Experience that Favours Inclusion of Screening for Irregular Erythrocyte Antibody in Routine Antenatal Testing Profile

Manoj Kahar  1
Affiliations

Frequency of Red Cell Alloantibodies in Pregnant Females of Navsari District: An Experience that Favours Inclusion of Screening for Irregular Erythrocyte Antibody in Routine Antenatal Testing Profile

Manoj Kahar. J Obstet Gynaecol India. 2018 Aug.

Abstract

Background: Alloimmunisation due to irregular erythrocyte antibodies is a recognised cause of hemolytic disease of the fetus and newborn (HDFN). Prior knowledge of red cell alloimmunisation in pregnant females guides the obstetrician to monitor the foetus for HDFN and if required for appropriated intervention. As limited data are available on prevalence of red cell alloimmunisation in pregnant females in India, the current study was carried out to know the prevalence of red cell alloimmunisation in pregnant females coming at our laboratory.

Methods: Screening for irregular erythrocyte antibodies was performed in 1960 pregnant females after obtaining informed consent between June 2015 and June 2016. MatrixTM screening and identification reagent red cells from Tulip Diagnostics (P) Ltd were used, and column agglutination technique was employed as a method for the test.

Results: Twenty antibodies (all of single specificity) were detected in 1960 samples giving a prevalence rate of alloimmunisation of 1.02%. Out of the 20 antibodies, 18 were identified to be anti-D, 1 was anti-c and 1 antibody was anti-H. The results obtained were then compared with those reported in the literature.

Conclusion: Red cell alloimmunisation is not uncommonly observed in pregnant females; the information gained can help the obstetrician to identify high-risk cases to timely start antenatal and post-natal treatment. Obstetricians should request screening for irregular red cell antibody desirably in all pregnant females; however, if limiting factors are there, it should be done at least in select group of pregnant females having bad obstetric history.

Keywords: Alloimmunisation; Antenatal; Antibody screening; HDFN; RBC antibody.

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Conflict of interest statement

Compliance with Ethical StandardsDr. Manoj Kahar declares that he has no conflict of interest and has not received any grants for the present study.Informed consent was obtained from all patients for being included in the study. All procedures for the study were in accordance with established ethical standards. No identifying information of any patient is included in this article.

Figures

Fig. 1
Fig. 1
Reagent red cells used for detection and identification of red cell alloantibodies
Fig. 2
Fig. 2
Equipments used for red cell alloantibody detection and identification
See this image and copyright information in PMC

References

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