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Comment
. 2018 Aug;59(2):271-273.
doi: 10.1165/rcmb.2018-0042LE.

Involvement of Microglial Cells in Hypoxia-induced Pulmonary Hypertension

Aline C Oliveira  1 , Ravindra K Sharma  1 , Victor Aquino  1 , Gilberto Lobaton  1 , Andrew J Bryant  1 , Jeffrey K Harrison  1 , Elaine M Richards  1 , Mohan K Raizada  1
Affiliations
Comment

Involvement of Microglial Cells in Hypoxia-induced Pulmonary Hypertension

Aline C Oliveira et al. Am J Respir Cell Mol Biol. 2018 Aug.
No abstract available

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Figures

Figure 1.
Figure 1.
Protection against hypoxia-induced pulmonary hypertension in CX3CR1-deficient mice correlates with decreased microglia activation. (A–D) Representative micrographs of immunohistochemical staining of microglia with an antibody against Iba1 using 3,3-diaminobenzidine detection in the paraventricular nucleus in wild-type (WT)-normoxia (A), CX3CR1-GFP/GFP- normoxia (B), WT-hypoxia (C), and CX3CR1-GFP/GFP-hypoxia (D) mice. Scale bars: 20 μm. (E) Chronic hypoxia induced an increase in the number of Iba1-positive cells (microglia) in WT mice, but not in CX3CR1GFP/GFP mice (*P < 0.05), and (F) a positive correlation between the number of microglia and the respective right ventricular systolic pressure (RVSP) was revealed (*P = 0.0057). Significant differences between groups were determined by one-way ANOVA followed by Newman-Keuls post hoc tests. Data are presented as mean ± SEM (n = 4/group). (G–J) Representative drawing of microglia captured in 100× images from WT-normoxia (G), CX3CR1-GFP/GFP-normoxia (H), WT-hypoxia (I), and CX3CR1-GFP/GFP-hypoxia (J) mice. Scale bars: 10 μm. H = hypoxia; Iba1 = ionized calcium-binding adaptor molecule 1; N = normoxia.
See this image and copyright information in PMC

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