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. 2018 Aug 2;19(1):576.
doi: 10.1186/s12864-018-4962-9.

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

Marcella Sabino  1 , Katia Cappelli  2 , Stefano Capomaccio  1 , Luisa Pascucci  1 , Ilaria Biasato  3 , Andrea Verini-Supplizi  1 , Andrea Valiani  4 , Massimo Trabalza-Marinucci  1
Affiliations

Dietary supplementation with olive mill wastewaters induces modifications on chicken jejunum epithelial cell transcriptome and modulates jejunum morphology

Marcella Sabino et al. BMC Genomics. .

Abstract

Background: The Mediterranean diet is considered one of the healthier food habits and olive oil is one of its key components. Olive oil polyphenols are known to induce beneficial effects in several pathological conditions, such as inflammatory bowel disease, and to contrast the proliferation of cancer cells or hypercholesterolemia. Polyphenols are also present in waste products derived from the olive industry: olive mill wastewaters (OMWW) are rich in polyphenols and there is an increasing interest in using OMWW in animal nutrition. OMWW are attributed with positive effects in promoting chicken performance and the quality of food-derived products. However, a tissue-specific transcriptome target analysis of chickens fed with OMWW has never been attempted.

Results: We explored the effect of dietary OMWW on the intestinal function in broilers. A morphological analysis of the jejunum revealed that OMWW reduced crypt depth, whereas no significant modifications were observed for villus height and the villus height/crypt depth ratio. An RNA Sequencing analysis was performed on isolated, intestinal, epithelial cells and 280 differentially expressed genes were found using a count-based approach. An enrichment analysis revealed that the majority of up regulated genes in the OMWW group were over-represented by the regulation of viral genome replication-related GO-Terms, whereas down regulated genes were mainly involved in cholesterol and lipid metabolism.

Conclusions: Our study showed how an industrial waste product can be recycled as a feed additive with a positive relapse. OMWW dietary supplementation can be a nutritional strategy to improve chicken performance and health, prevent intestinal damage, enhance innate immunity and regulate cholesterol metabolism and fat deposition.

Keywords: Anti-viral activity; Cholesterol biosynthesis; Differentially expressed genes; Fatty acid metabolism; Nutrigenomics.

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Conflict of interest statement

The study was conducted in accordance with the European recommendations for the protection of animals used for scientific purposes (EU Directive 2010/63/EU) and approved by the Italian Ministry of Health (Authorisation n. 506/2016 – PR).

Not applicable.

The authors declare that they have no competing interests.

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Light and transmission electron microscopy features of jejunum epithelial cells obtained by collagenase digestion. a and d. Single and grouped epithelial cells of control (a) and treated animals (d). Note the columnar shape and the typical eosinophilic brush border (arrow). Haematoxylin-Eosin, scale bar: 10 μm. b and e. Epithelial cell strips obtained by digestion of control (b) and treated jejunum tracts (e). The medium power view reveals the single layer of polarized columnar cells that cover the villous surface. The tall nuclei are lined up at the base of the cells while the apical surface is covered by microvilli (arrow). Transmission electron microscopy. Scale bar, 5000 nm. c and f. High power view of the luminal portion of the epithelial cells obtained by digestion of control (c) and treated jejunum tracts (f). Observe the surface of the columnar epithelial cells with the “brush border” consisting of closely packed microvilli. Transmission electron microscopy. Scale bar, 5000 nm
Fig. 2
Fig. 2
Experimental design and data analysis workflow
See this image and copyright information in PMC

References

    1. Corella D, Ordovás JM. How does the Mediterranean diet promote cardiovascular health? Current progress toward molecular mechanisms: gene-diet interactions at the genomic, transcriptomic, and epigenomic levels provide novel insights into new mechanisms. BioEssays. 2014;36:526–537. doi: 10.1002/bies.201300180. - DOI - PubMed
    1. Stefani M, Rigacci S. Beneficial properties of natural phenols: highlight on protection against pathological conditions associated with amyloid aggregation. Biofactors. 2014;40:482–493. doi: 10.1002/biof.1171. - DOI - PubMed
    1. Rigacci S, Stefani M. Nutraceutical Properties of Olive Oil Polyphenols. An Itinerary from Cultured Cells through Animal Models to Humans. Int J Mol Sci [Internet]. 2016 [cited 2017 Sep 12];17. Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926377/ - PMC - PubMed
    1. Servili M, Esposto S, Fabiani R, Urbani S, Taticchi A, Mariucci F, et al. Phenolic compounds in olive oil: antioxidant, health and organoleptic activities according to their chemical structure. Inflammopharmacology. 2009;17:76–84. doi: 10.1007/s10787-008-8014-y. - DOI - PubMed
    1. Tripoli E, Giammanco M, Tabacchi G, Di Majo D, Giammanco S, La Guardia M. The phenolic compounds of olive oil: structure, biological activity and beneficial effects on human health. Nutr Res Rev. 2005;18:98–112. doi: 10.1079/NRR200495. - DOI - PubMed

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