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. 2018 Aug;8(8):343.
doi: 10.1007/s13205-018-1361-5. Epub 2018 Jul 27.

wound healing activity of Morinda tinctoria Roxb aqueous leaf extract

S Rex Jeya Rajkumar  1   2 , G Gnanavel  2 , M S A Muthukumar Nadar  2 , Rajkumar Sankaranarayanan  3   4
Affiliations

wound healing activity of Morinda tinctoria Roxb aqueous leaf extract

S Rex Jeya Rajkumar et al. 3 Biotech. 2018 Aug.

Abstract

This study investigated the wound healing properties of the aqueous extract of Morinda tinctoria Roxb leaves in rats. The study also provides information on the purification, qualitative, and quantitative analysis of phytochemical components present in M. tinctoria Roxb. Wound contraction and period of epithelialization was determined and topical application of M. tinctoria Roxb aqueous leaf extract showed better healing than orally treated and control groups. The results suggest that the efficacy of M. tinctoria Roxb aqueous leaf extract as a wound healing agent and can also be used as a therapeutic agent for internal as well as external wounds.

Keywords: Aqueous extract; Morinda tinctoria Roxb; Phytochemicals; Wound healing.

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Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Phytochemical analysis of aqueous extract of M. tinctoria Roxb leaves using thin layer chromatography. Black and white arrow indicates the presence of corresponding phytochemical constituents. C authentic sample, M aqueous extract of M. tinctoria Roxb leaves
Fig. 2
Fig. 2
Thin layer chromatographic analysis of purified phytochemicals of aqueous leaf extract of M. tinctoria Roxb
Fig. 3
Fig. 3
Percentage of wound contraction of rats treated with M. tinctoria Roxb extract (Test O: oral administration of test rats with M. tinctoria Roxb leaf extract, Test L: local administration of test rats with M. tinctoria Roxb extract). Values are represented as mean ± SEM with triplicate estimations (**P < 0.01)
Fig. 4
Fig. 4
Period of epithelialization of rats treated with aqueous extract of M. tinctoria Roxb leaves
Fig. 5
Fig. 5
Activity of matrix metallo proteinases-2 (MMP-2) during wound healing (Test O: oral administration of test rats with M. tinctoria Roxb extract, Test L: local administration of test rats with M. tinctoria Roxb extracts). Values are expressed in mean ± SD
Fig. 6
Fig. 6
Activity of matrix metallo proteinases-9 (MMP-9) during wound healing (Test O: oral administration of test rats with aqueous extract of M. tinctoria Roxb leaves extract, Test L: local administration of test rats with aqueous extract of M. tinctoria Roxb leaves). Values are expressed in mean ± SD
Fig. 7
Fig. 7
SDS-PAGE gelatin zymography analysis of MMPs (MMP-2 and MMP-9). a–c Analysis of MMP-2 and -9 on day 3, day 10, and day 20, respectively. Lanes 1–3: control group, lanes 4–6: test group treated with aqueous extract of M. tinctoria Roxb leaves by oral administration, lanes 7–9: test group treated with aqueous extract of M. tinctoria Roxb leaves by local application. Black arrow indicates position of MMP-9 and red arrow indicates position of MMP-2
Fig. 8
Fig. 8
Changes in level of reduced glutathione (GSH) in rats treated with aqueous extract of M. tinctoria Roxb leaves
Fig. 9
Fig. 9
Changes in level of sulfhydryl groups (SH) in rats treated with aqueous extract of M. tinctoria Roxb leaves
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    1. Black and white arrow indicates the presence of corresponding phytochemical constituents. C: Authentic sample, M: Aqueous extract of Morina tinctoria Roxb leaves
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